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Revision as of 22:38, 28 June 2026
Nanopore sequencing
Nanopore sequencing is a sequencing approach in which individual DNA or RNA molecules pass through nanopores and produce signal changes that can be interpreted as sequence information.
Features
- Long reads that can help with genome assembly and structural variant detection.
- Real-time data generation and analysis.
- Direct DNA or RNA sensing in many workflows.
- Portable instruments suitable for field and rapid-response settings.
Common workflow
- Sample preparation and library construction.
- Sequencing on a nanopore device.
- Basecalling from raw signal to nucleotide sequence.
- Quality control and filtering.
- Alignment, assembly, variant calling, or metagenomic classification.
- Interpretation and reporting.