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<span id="Nanopore_sequencing"></span>
= Nanopore sequencing =
= 纳米孔测序 =
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<div lang="en" dir="ltr" class="mw-content-ltr">
'''纳米孔测序''' 是一种测序方法,其中单个 DNA 或 RNA 分子通过纳米孔并产生可以解释为序列信息的信号变化。
'''Nanopore sequencing''' is a sequencing approach in which individual DNA or RNA molecules pass through nanopores and produce signal changes that can be interpreted as sequence information.
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<div lang="en" dir="ltr" class="mw-content-ltr">
<span id="Features"></span>
== Features ==
== 特点 =
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<div lang="en" dir="ltr" class="mw-content-ltr">
* 长读长,有助于基因组组装和结构变异检测。
* Long reads that can help with genome assembly and structural variant detection.
* 实时数据生成和分析。
* Real-time data generation and analysis.
* 在许多工作流程中直接检测 DNA 或 RNA。
* Direct DNA or RNA sensing in many workflows.
* 便携式仪器,适用于现场和快速响应设置。
* Portable instruments suitable for field and rapid-response settings.
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<div lang="en" dir="ltr" class="mw-content-ltr">
<span id="Common_workflow"></span>
== Common workflow ==
== 常见工作流程 =
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<div lang="en" dir="ltr" class="mw-content-ltr">
# 样品制备和文库构建。
# Sample preparation and library construction.
# 在纳米孔设备上进行测序。
# Sequencing on a nanopore device.
# 从原始信号到核苷酸序列的碱基识别。
# Basecalling from raw signal to nucleotide sequence.
# 质量控制和过滤。
# Quality control and filtering.
# 比对、组装、变异检测或宏基因组分类。
# Alignment, assembly, variant calling, or metagenomic classification.
# 解释和报告。
# Interpretation and reporting.
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<div lang="en" dir="ltr" class="mw-content-ltr">
<span id="Related_pages"></span>
== Related pages ==
== 相关页面 =
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<div lang="en" dir="ltr" class="mw-content-ltr">
* [[Oxford Nanopore]]
* [[Oxford Nanopore]]
* [[MinION]]
* [[MinION]]
* [[Bioinformatics]]
* [[生物信息学]]
* [[Glossary]]
* [[术语表]]
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<languages />
<languages />

Revision as of 12:25, 29 June 2026

纳米孔测序

纳米孔测序 是一种测序方法,其中单个 DNA 或 RNA 分子通过纳米孔并产生可以解释为序列信息的信号变化。

= 特点

  • 长读长,有助于基因组组装和结构变异检测。
  • 实时数据生成和分析。
  • 在许多工作流程中直接检测 DNA 或 RNA。
  • 便携式仪器,适用于现场和快速响应设置。

= 常见工作流程

  1. 样品制备和文库构建。
  2. 在纳米孔设备上进行测序。
  3. 从原始信号到核苷酸序列的碱基识别。
  4. 质量控制和过滤。
  5. 比对、组装、变异检测或宏基因组分类。
  6. 解释和报告。

= 相关页面