Nanopore sequencing: Difference between revisions
Convert page to Translate-managed source |
Marked this version for translation |
||
| Line 1: | Line 1: | ||
<translate> | <translate> | ||
= Nanopore sequencing = | = Nanopore sequencing = <!--T:1--> | ||
<!--T:2--> | |||
'''Nanopore sequencing''' is a sequencing approach in which individual DNA or RNA molecules pass through nanopores and produce signal changes that can be interpreted as sequence information. | '''Nanopore sequencing''' is a sequencing approach in which individual DNA or RNA molecules pass through nanopores and produce signal changes that can be interpreted as sequence information. | ||
== Features == | == Features == <!--T:3--> | ||
<!--T:4--> | |||
* Long reads that can help with genome assembly and structural variant detection. | * Long reads that can help with genome assembly and structural variant detection. | ||
* Real-time data generation and analysis. | * Real-time data generation and analysis. | ||
| Line 11: | Line 13: | ||
* Portable instruments suitable for field and rapid-response settings. | * Portable instruments suitable for field and rapid-response settings. | ||
== Common workflow == | == Common workflow == <!--T:5--> | ||
<!--T:6--> | |||
# Sample preparation and library construction. | # Sample preparation and library construction. | ||
# Sequencing on a nanopore device. | # Sequencing on a nanopore device. | ||
| Line 20: | Line 23: | ||
# Interpretation and reporting. | # Interpretation and reporting. | ||
== Related pages == | == Related pages == <!--T:7--> | ||
<!--T:8--> | |||
* [[Oxford Nanopore]] | * [[Oxford Nanopore]] | ||
* [[MinION]] | * [[MinION]] | ||
Revision as of 22:38, 28 June 2026
Nanopore sequencing
Nanopore sequencing is a sequencing approach in which individual DNA or RNA molecules pass through nanopores and produce signal changes that can be interpreted as sequence information.
Features
- Long reads that can help with genome assembly and structural variant detection.
- Real-time data generation and analysis.
- Direct DNA or RNA sensing in many workflows.
- Portable instruments suitable for field and rapid-response settings.
Common workflow
- Sample preparation and library construction.
- Sequencing on a nanopore device.
- Basecalling from raw signal to nucleotide sequence.
- Quality control and filtering.
- Alignment, assembly, variant calling, or metagenomic classification.
- Interpretation and reporting.