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<div class="plainlinks">'''Languages:''' [[Nanopore sequencing|English]] | [[Nanopore sequencing/zh|中文]] | [[Nanopore sequencing/es|español]] | [[Nanopore sequencing/fr|français]] | [[Nanopore sequencing/de|Deutsch]] | [[Nanopore sequencing/ja|日本語]]</div>
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= Nanopore sequencing =
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= ナノポアシークエンシング =
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'''Nanopore sequencing''' is a sequencing approach in which individual DNA or RNA molecules pass through nanopores and produce signal changes that can be interpreted as sequence information.
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'''ナノポアシークエンシング'''は、個々のDNAやRNA分子がナノポアを通過し、その信号の変化をシークエンス情報として解釈するシークエンシング手法です。
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== Features ==
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== 特徴 ==
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* Long reads that can help with genome assembly and structural variant detection.
* Real-time data generation and analysis.
* Direct DNA or RNA sensing in many workflows.
* Portable instruments suitable for field and rapid-response settings.
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* ゲノムアセンブリや構造変異検出に役立つ長いリード。
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* リアルタイムのデータ生成と分析。
== Common workflow ==
* 多くのワークフローで直接DNAやRNAを検出。
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* 携帯可能な機器で、現場や迅速な対応に適しています。


== 一般的なワークフロー ==
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# Sample preparation and library construction.
# Sequencing on a nanopore device.
# Basecalling from raw signal to nucleotide sequence.
# Quality control and filtering.
# Alignment, assembly, variant calling, or metagenomic classification.
# Interpretation and reporting.
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# サンプル準備とライブラリ構築。
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# ナノポアデバイスでのシークエンシング。
== Related pages ==
# 生の信号からヌクレオチドシークエンスへのベースコール。
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# 品質管理とフィルタリング。
# アライメント、アセンブリ、変異コール、またはメタゲノム分類。
# 解釈と報告。


== 関連ページ ==
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* [[Oxford Nanopore]]
* [[MinION]]
* [[Bioinformatics]]
* [[Glossary]]
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* [[Oxford Nanopore|Oxford Nanopore]]
<languages />
* [[MinION|MinION]]
* [[Bioinformatics|バイオインフォマティクス]]
* [[Glossary|用語集]]

Revision as of 23:06, 28 June 2026

Nanopore sequencing

Nanopore sequencing is a sequencing approach in which individual DNA or RNA molecules pass through nanopores and produce signal changes that can be interpreted as sequence information.

Features

  • Long reads that can help with genome assembly and structural variant detection.
  • Real-time data generation and analysis.
  • Direct DNA or RNA sensing in many workflows.
  • Portable instruments suitable for field and rapid-response settings.

Common workflow

  1. Sample preparation and library construction.
  2. Sequencing on a nanopore device.
  3. Basecalling from raw signal to nucleotide sequence.
  4. Quality control and filtering.
  5. Alignment, assembly, variant calling, or metagenomic classification.
  6. Interpretation and reporting.