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<div class="plainlinks">'''Languages:''' [[Nanopore sequencing|English]] | [[Nanopore sequencing/zh|中文]] | [[Nanopore sequencing/es|español]] | [[Nanopore sequencing/fr|français]] | [[Nanopore sequencing/de|Deutsch]] | [[Nanopore sequencing/ja|日本語]]</div>
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= Nanopore sequencing =
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= 纳米孔测序 =
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'''Nanopore sequencing''' is a sequencing approach in which individual DNA or RNA molecules pass through nanopores and produce signal changes that can be interpreted as sequence information.
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'''纳米孔测序'''是一种测序方法,其中单个DNA或RNA分子通过纳米孔并产生可以解释为序列信息的信号变化。
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== Features ==
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== 特点 ==
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* Long reads that can help with genome assembly and structural variant detection.
* Real-time data generation and analysis.
* Direct DNA or RNA sensing in many workflows.
* Portable instruments suitable for field and rapid-response settings.
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* 长读长,有助于基因组组装和结构变异检测。
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* 实时数据生成和分析。
== Common workflow ==
* 在许多工作流程中直接检测DNA或RNA。
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* 便携式仪器,适用于现场和快速响应设置。


== 常见工作流程 ==
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# Sample preparation and library construction.
# Sequencing on a nanopore device.
# Basecalling from raw signal to nucleotide sequence.
# Quality control and filtering.
# Alignment, assembly, variant calling, or metagenomic classification.
# Interpretation and reporting.
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# 样本准备和文库构建。
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# 在纳米孔设备上进行测序。
== Related pages ==
# 从原始信号到核苷酸序列的碱基识别。
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# 质量控制和过滤。
# 比对、组装、变异检测或宏基因组分类。
# 解释和报告。


== 相关页面 ==
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* [[Oxford Nanopore]]
* [[MinION]]
* [[Bioinformatics]]
* [[Glossary]]
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* [[Oxford Nanopore|Oxford Nanopore]]
<languages />
* [[MinION|MinION]]
* [[生物信息学]]
* [[术语表]]

Revision as of 23:45, 28 June 2026

Nanopore sequencing

Nanopore sequencing is a sequencing approach in which individual DNA or RNA molecules pass through nanopores and produce signal changes that can be interpreted as sequence information.

Features

  • Long reads that can help with genome assembly and structural variant detection.
  • Real-time data generation and analysis.
  • Direct DNA or RNA sensing in many workflows.
  • Portable instruments suitable for field and rapid-response settings.

Common workflow

  1. Sample preparation and library construction.
  2. Sequencing on a nanopore device.
  3. Basecalling from raw signal to nucleotide sequence.
  4. Quality control and filtering.
  5. Alignment, assembly, variant calling, or metagenomic classification.
  6. Interpretation and reporting.