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Glossary

From Nanopore Wiki
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File:202001 nanopore sequencing.svg
Principle of nanopore sequencing. Source: Wikimedia Commons, CC BY 4.0.

Glossary is part of the Wiki learning path. This glossary defines common terms used in nanopore technology, sequencing, signal analysis, and experimental workflows.

Core concepts

  • Open-pore current: baseline current through an unoccupied pore.
  • Blockade depth: the magnitude of current reduction or signal shift during an event.
  • Dwell time: the duration for which a molecule produces a detectable event.
  • Basecalling: conversion of nanopore raw signal into nucleotide sequence and quality values.
  • POD5, FAST5, FASTQ, and BAM: common file formats in Oxford Nanopore workflows.

How to use this page

Step Description
1 Use definitions to keep wording consistent across pages.
2 Prefer exact terms such as open-pore current, blockade event, and Q score.
3 When a term has platform-specific meaning, state the platform explicitly.

Typical applications

  • Quick reference for new readers.
  • Editorial consistency across translated pages.
  • Support for classroom and laboratory onboarding.

Common pitfalls

  • Using sequencing terms for non-sequencing nanopore experiments without qualification.
  • Translating file formats or instrument names that should remain unchanged.

References