Single-molecule detection
Single-molecule detection
Single-molecule detection refers to methods that observe individual molecules rather than measuring only bulk averages. Nanopores are useful for single-molecule sensing because each molecule can produce a distinct signal while interacting with the pore.
What can be detected
- DNA and RNA molecules.
- Peptides and proteins.
- Small molecules and metabolites.
- Biomarkers related to disease or environmental monitoring.
Why it matters
Single-molecule methods can reveal heterogeneity, rare events, molecular modifications, and transient interactions that may be hidden in ensemble measurements.