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= Welcome to Nanopore Wiki =
__NOTOC__
 
<div style="border:1px solid #a2a9b1; background:#f8f9fa; padding:1.2em; margin-bottom:1em; overflow:hidden;">
[[File:Nanopore_sequencing_principle.png|thumb|right|360px|Principle of nanopore sequencing. Source: Wikimedia Commons, DBCLS, CC BY 4.0.]]
[[File:Nanopore_sequencing_principle.png|thumb|right|360px|Principle of nanopore sequencing. Source: Wikimedia Commons, DBCLS, CC BY 4.0.]]
<div style="font-size:188%; line-height:1.2; margin-bottom:0.25em;">Welcome to Nanopore Wiki</div>
<div style="font-size:108%; max-width:58em;">A knowledge base and science outreach site for nanopore technology, nanopore sequencing, single-molecule sensing, bioinformatics workflows, instruments, and frontier research.</div>
</div>


'''Nanopore Wiki''' is a knowledge base and science outreach site focused on nanopore technology and frontier science. The site covers nanopore sequencing, single-molecule detection, bioinformatics, experimental workflows, instrument platforms, research progress, and related foundational concepts. Its goal is to organize scattered technical materials into a clear, searchable, and continuously updated knowledge system.
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== What Is a Nanopore? ==
== Start Here ==
 
A '''nanopore''' usually refers to a nanoscale pore. It may exist in biological membrane proteins or be fabricated from solid-state materials. The core idea is that when ions, DNA, RNA, proteins, or other molecules pass through an extremely small pore, they change the electrical current, ion flow, or other measurable signals in the pore. Researchers can use these signal changes to infer a molecule's size, structure, composition, or movement.
 
Nanopores are not only used for DNA or RNA sequencing. They can also be used to detect small molecules, peptides, proteins, biopolymers, carbohydrates, and disease biomarkers, with potential applications in environmental monitoring, food safety, metabolite analysis, and other fields.
 
== What Is Nanopore Sequencing? ==
 
'''Nanopore sequencing''' is a type of third-generation sequencing technology. It typically allows a single DNA or RNA molecule to pass through a nanopore and measures the changes in electrical signals as the molecule moves through the pore, making it possible to infer the nucleic acid sequence.
 
Compared with many traditional sequencing methods, nanopore sequencing has several distinctive features:
 
* It can directly read single DNA or RNA molecules.
* In many scenarios, it does not require PCR amplification or fluorescent labeling.
* Devices can be miniaturized and made portable, making them suitable for field detection.
* Data can be generated and analyzed in real time.
* Long-read capability is useful for genome assembly, structural variant analysis, and haplotype studies.
 
== Typical Applications ==
 
* '''Pathogen detection''': Rapid identification of viruses, bacteria, and other pathogens.
* '''Public health surveillance''': For example, variant tracking and wastewater monitoring during outbreaks.
* '''Environmental monitoring''': Research on environmental DNA, microbial communities, and pollution-related questions.
* '''Agriculture and food safety''': Crop disease detection, food contamination analysis, and traceability.
* '''Genome research''': Sequencing of human, animal, plant, and microbial genomes.
* '''Epigenetics and modification detection''': Exploration of methylation and other base modification signals.
* '''Single-molecule sensing''': Extension to proteins, small molecules, and biomarkers.
 
== Site Positioning ==
 
* '''Knowledge base''': A place to collect searchable and updatable topic pages and learning materials.
* '''Science outreach site''': Clear and accurate explanations of nanopore technology and its applications.
* '''Frontier watch''': Tracking important papers, tools, methods, and industry developments.
* '''Practical reference''': Notes on experimental workflows, data analysis approaches, and common questions.
 
== Recommended Entry Points ==
 
* [[Nanopore technology]]
* [[Nanopore technology]]
* [[Nanopore sequencing]]
* [[Nanopore sequencing]]
* [[Single-molecule detection]]
* [[Single-molecule detection]]
* [[Bioinformatics]]
* [[Bioinformatics]]
* [[Oxford Nanopore]]
* [[MinION]]
* [[Research progress]]
* [[Glossary]]
* [[Glossary]]


== Editorial Principles ==
Nanopores are nanoscale pores that can be used to observe molecules through changes in electrical current, ion flow, or other measurable signals. This makes them useful for sequencing, molecular sensing, and real-time analysis.


* Base content on reliable sources and avoid unverified claims.
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* Distinguish facts, explanations, hypotheses, and opinions.
== Latest Progress ==
* Encourage continuous revision so pages improve as knowledge evolves.
{{:Latest Progress}}
* Serve readers in clear English while preserving technical accuracy for specialist users.
|-
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== Research Topics ==
* [[Long-read sequencing]]
* [[Direct RNA sequencing]]
* [[Epigenetic modification detection]]
* [[Pathogen detection]]
* [[Environmental DNA]]
* [[Protein nanopore sensing]]
 
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== Tools and Workflows ==
* [[MinION]]
* [[Oxford Nanopore]]
* [[Basecalling]]
* [[Read mapping]]
* [[Variant calling]]
* [[Genome assembly]]
* [[Quality control]]
|-
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== Applications ==
* Public health surveillance
* Clinical and pathogen genomics
* Agriculture and food safety
* Environmental monitoring
* Microbiome and metagenomics
* Structural variant analysis
 
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== Contribute ==
Add new pages, improve definitions, summarize recent papers, and keep methods pages up to date. For new progress items, edit [[Latest Progress]] so the homepage updates automatically.


== References ==
== References ==
* [https://en.wikipedia.org/wiki/Nanopore Nanopore - Wikipedia]
* [https://en.wikipedia.org/wiki/Nanopore Nanopore - Wikipedia]
* [https://en.wikipedia.org/wiki/Nanopore_sequencing Nanopore sequencing - Wikipedia]
* [https://en.wikipedia.org/wiki/Nanopore_sequencing Nanopore sequencing - Wikipedia]
* [https://commons.wikimedia.org/wiki/File:202001_nanopore_sequencing.svg Principle of nanopore sequencing - Wikimedia Commons]
* [https://commons.wikimedia.org/wiki/File:202001_nanopore_sequencing.svg Principle of nanopore sequencing - Wikimedia Commons]
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Revision as of 12:41, 27 June 2026

Principle of nanopore sequencing. Source: Wikimedia Commons, DBCLS, CC BY 4.0.
Welcome to Nanopore Wiki
A knowledge base and science outreach site for nanopore technology, nanopore sequencing, single-molecule sensing, bioinformatics workflows, instruments, and frontier research.

Start Here

Nanopores are nanoscale pores that can be used to observe molecules through changes in electrical current, ion flow, or other measurable signals. This makes them useful for sequencing, molecular sensing, and real-time analysis.

Latest Progress

  • 2026-06-27 - Nanopore Wiki homepage launched with a portal-style layout for tracking nanopore technology, sequencing, tools, and research progress.


Research Topics

Tools and Workflows

Applications

  • Public health surveillance
  • Clinical and pathogen genomics
  • Agriculture and food safety
  • Environmental monitoring
  • Microbiome and metagenomics
  • Structural variant analysis

Contribute

Add new pages, improve definitions, summarize recent papers, and keep methods pages up to date. For new progress items, edit Latest Progress so the homepage updates automatically.

References

Retrieved from "https://wiki.nanopore.net/index.php?title=Main_Page&oldid=9"