MinION: Difference between revisions

Revision as of 10:12, 30 June 2026

MinION is part of the Nanopore Wiki learning path. MinION is a portable nanopore sequencing device associated with Oxford Nanopore Technologies and widely used for field, teaching, and laboratory sequencing.

Core concepts

MinION uses disposable flow cells with many nanopore channels.
It supports real-time sequencing and portable workflows.
Run success depends on sample quality, library preparation, flow cell state, and software configuration.
Downstream interpretation requires appropriate bioinformatics and quality control.

Practical workflow

Step	Description
1	Check flow cell status and plan library input.
2	Prepare and load the sequencing library.
3	Monitor run metrics such as active pores, yield, read length, and quality.
4	Analyze reads with a documented pipeline.

Typical applications

Rapid microbial and viral sequencing.
Teaching long-read sequencing concepts.
Field and resource-limited deployments.
Small genome assembly and targeted sequencing.

Common pitfalls

Assuming portability removes the need for sample quality control.
Using old basecaller models without recording versions.
Ignoring contamination and negative controls in field workflows.

@@ Line 1: / Line 1: @@
+__NOTOC__
 <translate>
-= MinION = <!--T:1-->
+<!--T:1-->
+[[File:Nanopore_sequencing_principle.png|thumb|right|320px|Nanopore sequencing principle. Openly licensed schematic used as a general visual guide.]]
+'''MinION''' is part of the Nanopore Wiki learning path. MinION is a portable nanopore sequencing device associated with Oxford Nanopore Technologies and widely used for field, teaching, and laboratory sequencing.
 <!--T:2-->
-'''MinION''' is a portable nanopore sequencing device associated with Oxford Nanopore Technologies. It is often used in field sequencing, rapid pathogen surveillance, education, and small-scale sequencing workflows.
+== Core concepts ==
+* MinION uses disposable flow cells with many nanopore channels.
+* It supports real-time sequencing and portable workflows.
+* Run success depends on sample quality, library preparation, flow cell state, and software configuration.
+* Downstream interpretation requires appropriate bioinformatics and quality control.
-== Typical uses == <!--T:3-->
+<!--T:3-->
+== Practical workflow ==
+{| class="wikitable np-knowledge-table"
+! Step
+! Description
+|-
+| 1
+| Check flow cell status and plan library input.
+|-
+| 2
+| Prepare and load the sequencing library.
+|-
+| 3
+| Monitor run metrics such as active pores, yield, read length, and quality.
+|-
+| 4
+| Analyze reads with a documented pipeline.
+|}
 <!--T:4-->
-* Rapid microbial or viral sequencing.
+== Typical applications ==
-* Field and point-of-need genomics.
+* Rapid microbial and viral sequencing.
-* Teaching and demonstration of nanopore sequencing.
+* Teaching long-read sequencing concepts.
-* Pilot experiments before scaling to larger platforms.
+* Field and resource-limited deployments.
+* Small genome assembly and targeted sequencing.
-== Related pages == <!--T:5-->
+<!--T:5-->
+== Common pitfalls ==
+* Assuming portability removes the need for sample quality control.
+* Using old basecaller models without recording versions.
+* Ignoring contamination and negative controls in field workflows.
 <!--T:6-->
+== Related pages ==
 * [[Oxford Nanopore]]
 * [[Nanopore sequencing]]
 * [[Bioinformatics]]
+* [[Experimental workflows]]
+<!--T:7-->
+== References ==
+* [https://en.wikipedia.org/wiki/MinION MinION]
+* [https://nanoporetech.com/ Oxford Nanopore Technologies official site]
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