Nanopore technology - Revision history

Nano: Expand Nanopore Wiki source page with structured educational content

2026-07-15T01:24:38Z

Expand Nanopore Wiki source page with structured educational content

← Older revision		Revision as of 09:24, 15 July 2026
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	<!--T:1-->		<!--T:1-->
	[[File:Nanopore_sequencing_principle.png\|thumb\|right\|320px\|~~Principle of nanopore sequencing. Source: Wikimedia Commons, CC BY 4.0~~.]]		[[File:Nanopore_sequencing_principle.png\|thumb\|right\|320px\|Nanopore detection converts molecular motion into measurable signal changes.]]
	'''Nanopore technology''' is part of the Nanopore Wiki learning path. Nanopore technology uses nanometer-scale openings to detect molecules through changes in ionic current, tunneling current, optical signal, or other measurable readouts.		'''Nanopore technology''' is part of the Nanopore Wiki learning path. Nanopore technology uses nanometer-scale openings to detect molecules through changes in ionic current, tunneling current, optical signal, or other measurable readouts.

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			== Scientific basis ==
			* The pore geometry defines the sensing volume and constrains which molecules can be resolved.
			* Biological pores offer reproducible atomic structures; solid-state pores offer material and device flexibility.
			* Interpretations should be tied to measurable quantities, experimental controls, and documented analysis settings.
			* Claims should distinguish direct observations from downstream inference.

			<!--T:4-->
	== How a measurement is organized ==		== How a measurement is organized ==
	{\| class="wikitable np-knowledge-table"		{\| class="wikitable np-knowledge-table"
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	\|}		\|}

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	== Typical applications ==		== Typical applications ==
	* DNA and RNA sequencing.		* DNA and RNA sequencing.
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	* Portable, real-time molecular analysis.		* Portable, real-time molecular analysis.

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	== ~~Common pitfalls~~ ==		== Quality checks ==
			* Record platform, pore or flow-cell type, chemistry, voltage or acquisition settings, and software versions.
			* Use negative controls, positive controls, and replicate measurements where the claim depends on specificity.
			* Report limitations such as noise, bias, sample degradation, pore instability, or model-dependent analysis.
			* Use the related pages to move from introductory terms to experimental or computational detail.

			<!--T:7-->
			== Interpretation limits ==
	* Assuming all nanopores behave like commercial sequencing flow cells.		* Assuming all nanopores behave like commercial sequencing flow cells.
	* Ignoring surface adsorption and non-specific interactions.		* Ignoring surface adsorption and non-specific interactions.
	* ~~Comparing data across platforms without matching bandwidth~~, ~~voltage~~, ~~buffer~~, and ~~pore size~~.		* A single result should not be generalized beyond the sample, protocol, instrument state, and analysis pipeline used.
			* Medical, legal, regulatory, or commercial decisions require domain-specific validation beyond this educational summary.

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	== Related pages ==		== Related pages ==
	* [[Introduction]]		* [[Introduction]]
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	* [[Nanopore sequencing]]		* [[Nanopore sequencing]]

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	== References ==		== References ==
	* [https://en.wikipedia.org/wiki/Nanopore Nanopore]		* [https://en.wikipedia.org/wiki/Nanopore Nanopore]

Nano: Use topic-specific Wikimedia Commons illustrations

2026-07-02T08:47:23Z

Use topic-specific Wikimedia Commons illustrations

← Older revision		Revision as of 16:47, 2 July 2026
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	[[File:~~202001 nanopore sequencing~~.~~svg~~\|thumb\|right\|320px\|Principle of nanopore sequencing. Source: Wikimedia Commons, CC BY 4.0.]]		[[File:Nanopore_sequencing_principle.png\|thumb\|right\|320px\|Principle of nanopore sequencing. Source: Wikimedia Commons, CC BY 4.0.]]
	'''Nanopore technology''' is part of the Nanopore Wiki learning path. Nanopore technology uses nanometer-scale openings to detect molecules through changes in ionic current, tunneling current, optical signal, or other measurable readouts.		'''Nanopore technology''' is part of the Nanopore Wiki learning path. Nanopore technology uses nanometer-scale openings to detect molecules through changes in ionic current, tunneling current, optical signal, or other measurable readouts.

Nano: Restore visible brand name to Nanopore Wiki

2026-06-30T03:47:15Z

Restore visible brand name to Nanopore Wiki

← Older revision		Revision as of 11:47, 30 June 2026
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	[[File:202001 nanopore sequencing.svg\|thumb\|right\|320px\|Principle of nanopore sequencing. Source: Wikimedia Commons, CC BY 4.0.]]		[[File:202001 nanopore sequencing.svg\|thumb\|right\|320px\|Principle of nanopore sequencing. Source: Wikimedia Commons, CC BY 4.0.]]
	'''Nanopore technology''' is part of the Wiki learning path. Nanopore technology uses nanometer-scale openings to detect molecules through changes in ionic current, tunneling current, optical signal, or other measurable readouts.		'''Nanopore technology''' is part of the Nanopore Wiki learning path. Nanopore technology uses nanometer-scale openings to detect molecules through changes in ionic current, tunneling current, optical signal, or other measurable readouts.

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Nano: Shorten visible site name to Wiki

2026-06-30T03:42:19Z

Shorten visible site name to Wiki

← Older revision		Revision as of 11:42, 30 June 2026
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	[[File:202001 nanopore sequencing.svg\|thumb\|right\|320px\|Principle of nanopore sequencing. Source: Wikimedia Commons, CC BY 4.0.]]		[[File:202001 nanopore sequencing.svg\|thumb\|right\|320px\|Principle of nanopore sequencing. Source: Wikimedia Commons, CC BY 4.0.]]
	'''Nanopore technology''' is part of the ~~Nanopore~~ Wiki learning path. Nanopore technology uses nanometer-scale openings to detect molecules through changes in ionic current, tunneling current, optical signal, or other measurable readouts.		'''Nanopore technology''' is part of the Wiki learning path. Nanopore technology uses nanometer-scale openings to detect molecules through changes in ionic current, tunneling current, optical signal, or other measurable readouts.

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Nano: Use topic-specific Wikimedia Commons illustrations

2026-06-30T02:56:42Z

Use topic-specific Wikimedia Commons illustrations

← Older revision		Revision as of 10:56, 30 June 2026
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	[[File:~~Nanopore_sequencing_principle~~.~~png~~\|thumb\|right\|320px\|~~Nanopore~~ sequencing ~~principle~~. ~~Openly licensed schematic used as a general visual guide~~.]]		[[File:202001 nanopore sequencing.svg\|thumb\|right\|320px\|Principle of nanopore sequencing. Source: Wikimedia Commons, CC BY 4.0.]]
	'''Nanopore technology''' is part of the Nanopore Wiki learning path. Nanopore technology uses nanometer-scale openings to detect molecules through changes in ionic current, tunneling current, optical signal, or other measurable readouts.		'''Nanopore technology''' is part of the Nanopore Wiki learning path. Nanopore technology uses nanometer-scale openings to detect molecules through changes in ionic current, tunneling current, optical signal, or other measurable readouts.

Nano: Expand Nanopore Wiki source page with structured educational content

2026-06-30T02:10:03Z

Expand Nanopore Wiki source page with structured educational content

← Older revision		Revision as of 10:10, 30 June 2026
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			__NOTOC__
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	~~= Nanopore technology =~~ <!--T:1-->		<!--T:1-->
			[[File:Nanopore_sequencing_principle.png\|thumb\|right\|320px\|Nanopore sequencing principle. Openly licensed schematic used as a general visual guide.]]
			'''Nanopore technology''' is part of the Nanopore Wiki learning path. Nanopore technology uses nanometer-scale openings to detect molecules through changes in ionic current, tunneling current, optical signal, or other measurable readouts.

	<!--T:2-->		<!--T:2-->
	~~'''Nanopore technology''' uses nanoscale pores to observe~~ molecules ~~through changes in electrical current, ion flow, or related measurable signals. A nanopore~~ can be ~~biological, such as a protein pore embedded in a membrane, or~~ solid-state, ~~fabricated from materials such as silicon nitride~~ or ~~graphene~~.		== Core concepts ==
			* The pore geometry defines the sensing volume and constrains which molecules can be resolved.
			* Biological pores offer reproducible atomic structures; solid-state pores offer material and device flexibility.
			* Signal quality depends on pore state, membrane or chip stability, electronics, chemistry, and analysis.
			* Sequencing is one engineered application of a broader nanopore detection principle.

	== ~~Core idea~~ == <!--T:3-->		<!--T:3-->
			== How a measurement is organized ==
			{\| class="wikitable np-knowledge-table"
			! Step
			! Description
			\|-
			\| 1
			\| Choose the pore system and define the target molecule.
			\|-
			\| 2
			\| Prepare buffer, sample, membrane or chip, and low-noise recording conditions.
			\|-
			\| 3
			\| Record an open-pore baseline and verify that the system is stable.
			\|-
			\| 4
			\| Add sample, detect events, and compare with controls and metadata.
			\|}

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	~~When a molecule passes through or interacts with a nanopore~~, ~~it partially blocks or modifies the signal across the pore. The resulting signal pattern can provide information about molecular size~~, ~~charge~~, ~~structure~~, ~~sequence~~, ~~or binding behavior~~.		== Typical applications ==
			* DNA and RNA sequencing.
			* Protein, peptide, polymer, particle, and small-molecule sensing.
			* Single-channel biophysics and pore engineering.
			* Portable, real-time molecular analysis.

	~~== Major directions ==~~ <!--T:5-->		<!--T:5-->
			== Common pitfalls ==
			* Assuming all nanopores behave like commercial sequencing flow cells.
			* Ignoring surface adsorption and non-specific interactions.
			* Comparing data across platforms without matching bandwidth, voltage, buffer, and pore size.

	<!--T:6-->		<!--T:6-->
	* [[~~Nanopore sequencing~~]] ~~for DNA and RNA analysis.~~		== Related pages ==
	* [[~~Single-molecule detection~~]] ~~for proteins, peptides, small molecules, and biomarkers.~~		* [[Introduction]]
	* ~~Portable sensing systems for field, clinical, environmental, and food~~-~~safety use cases.~~		* [[Biological nanopores]]
	* ~~Long-read genomics and real-time data analysis workflows.~~		* [[Solid-state nanopores]]
			* [[Nanopore sequencing]]

	~~== Related pages ==~~ <!--T:7-->		<!--T:7-->
			== References ==
	~~<!--T:8-->~~		* [https://en.wikipedia.org/wiki/Nanopore Nanopore]
	* [[Nanopore ~~sequencing]~~]		* [https://commons.wikimedia.org/wiki/File:202001_nanopore_sequencing.svg Wikimedia Commons nanopore sequencing schematic]
	* [~~[Single-molecule detection]]~~
	* [[Bioinformatics]]
	* [[Glossary]]
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Nano: Marked this version for translation

2026-06-28T14:38:39Z

Marked this version for translation

← Older revision		Revision as of 22:38, 28 June 2026
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	= Nanopore technology =		= Nanopore technology = <!--T:1-->

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	'''Nanopore technology''' uses nanoscale pores to observe molecules through changes in electrical current, ion flow, or related measurable signals. A nanopore can be biological, such as a protein pore embedded in a membrane, or solid-state, fabricated from materials such as silicon nitride or graphene.		'''Nanopore technology''' uses nanoscale pores to observe molecules through changes in electrical current, ion flow, or related measurable signals. A nanopore can be biological, such as a protein pore embedded in a membrane, or solid-state, fabricated from materials such as silicon nitride or graphene.

	== Core idea ==		== Core idea == <!--T:3-->

			<!--T:4-->
	When a molecule passes through or interacts with a nanopore, it partially blocks or modifies the signal across the pore. The resulting signal pattern can provide information about molecular size, charge, structure, sequence, or binding behavior.		When a molecule passes through or interacts with a nanopore, it partially blocks or modifies the signal across the pore. The resulting signal pattern can provide information about molecular size, charge, structure, sequence, or binding behavior.

	== Major directions ==		== Major directions == <!--T:5-->

			<!--T:6-->
	* [[Nanopore sequencing]] for DNA and RNA analysis.		* [[Nanopore sequencing]] for DNA and RNA analysis.
	* [[Single-molecule detection]] for proteins, peptides, small molecules, and biomarkers.		* [[Single-molecule detection]] for proteins, peptides, small molecules, and biomarkers.
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	* Long-read genomics and real-time data analysis workflows.		* Long-read genomics and real-time data analysis workflows.

	== Related pages ==		== Related pages == <!--T:7-->

			<!--T:8-->
	* [[Nanopore sequencing]]		* [[Nanopore sequencing]]
	* [[Single-molecule detection]]		* [[Single-molecule detection]]

Nano: Convert page to Translate-managed source

2026-06-28T14:38:39Z

Convert page to Translate-managed source

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	<~~div class="plainlinks"~~>'''Languages:''' [[Nanopore technology\|English]] \| [[Nanopore technology/zh\|中文]] \| [[Nanopore technology/es\|español]] \| [[Nanopore technology/fr\|français]] \| [[Nanopore technology/de\|Deutsch]] \| [[Nanopore technology/ja\|日本語]]</div>		<translate>

	= Nanopore technology =		= Nanopore technology =

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	* [[Bioinformatics]]		* [[Bioinformatics]]
	* [[Glossary]]		* [[Glossary]]
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Nano: Fix language navigation encoding and localized link labels

2026-06-28T13:58:50Z

Fix language navigation encoding and localized link labels

← Older revision		Revision as of 21:58, 28 June 2026
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	<div class="plainlinks">'''Languages:''' [[Nanopore technology\|English]] \| [[Nanopore technology/zh\|??]] \| [[Nanopore technology/es\|~~espa?ol~~]] \| [[Nanopore technology/fr\|~~fran?ais~~]] \| [[Nanopore technology/de\|Deutsch]] \| [[Nanopore technology/ja\|~~???~~]]</div>		<div class="plainlinks">'''Languages:''' [[Nanopore technology\|English]] \| [[Nanopore technology/zh\|中文]] \| [[Nanopore technology/es\|español]] \| [[Nanopore technology/fr\|français]] \| [[Nanopore technology/de\|Deutsch]] \| [[Nanopore technology/ja\|日本語]]</div>

	= Nanopore technology =		= Nanopore technology =

Nano: Add language navigation

2026-06-28T13:48:02Z

Add language navigation

← Older revision		Revision as of 21:48, 28 June 2026
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			<div class="plainlinks">'''Languages:''' [[Nanopore technology\|English]] \| [[Nanopore technology/zh\|??]] \| [[Nanopore technology/es\|espa?ol]] \| [[Nanopore technology/fr\|fran?ais]] \| [[Nanopore technology/de\|Deutsch]] \| [[Nanopore technology/ja\|???]]</div>

	= Nanopore technology =		= Nanopore technology =